rluB [2017-11-27 17:58:06]

pseudouridine synthase

locus: BSU23160

pI: 10.25

mw: 25.88 kDa

protein length: 229 aa

gene length: 687 bp

function: 23S rRNA modification

product: pseudouridine synthase

essential: no

ec: 5.4.99.22

synonyms: ypuL

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

Gene

Coordinates: 2,421,438 → 2,422,172

The protein

Catalyzed reaction/ biological activity

• rRNA uridine = rRNA pseudouridine (according to Swiss-Prot)

Protein family

• pseudouridine synthase rsuA family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|YtzF]

Structure

• [PDB|4LAB] (from ''E. coli'', 40% identity) [Pubmed|24214967]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]-[gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]-[gene|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]-[gene|97FE84CF968596AC39D19EE2E43DA625147FD702|resC]-[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A106 (ypuL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/106 NBRP B. subtilis, Japan]
• BKE23160 (Δ[gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA, downstream forward: _UP4_TAAATTTCACATAACCTTCA
• BKK23160 (Δ[gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA, downstream forward: _UP4_TAAATTTCACATAACCTTCA

References

• 9888802,24214967